This is the second edition of a highly successful textbook (over 50,000 copies sold) in which a highly illustrated, narrative text is combined with easy-to-use thoroughly reliable laboratory protocols. It contains a fully up-to-date collection of 12 rigorously tested and reliable lab experiments in molecular biology, developed at the internationally renowned Dolan DNA Learning Center of Cold Spring Harbor Laboratory, which culminate in the construction and cloning of a recombinant DNA molecule.
'DNA Science' may be ordered direct from the Cold Spring Harbor Laboratory Press Web site: www.cshlpress.com
The UK Department of Trade and Industry and the Department of Health produced a pair of CD-ROMs for schools called 'Window on Life'. These were originally distributed with the Sunday Times newspaper on 16 November and 23 November 2003.
Additional copies of the CD-ROMs may be ordered free-of-charge from the 'Window on Life' Web site: www.windowonlife.org
This BAFTA award-winning DVD was a joint production of the Dolan DNA Learning Center, the Red Green and Blue Company Ltd and Windfall Films Ltd. The molecular animations were made by Drew Berry at the Walter and Eliza Hall Institute. Other animations were by Oscar-winning post production company The Mill (also known for their special effects in Harry Potter and Dr Who).
The disc contains 200 video clips and animations, providing approximately 4 hours of viewing. The DVD complements an excellent Web site of the same name: www.dnai.org
These five 50-minute documentaries were made by Windfall Films Ltd. They were broadcast to coincide with the 50th anniversary of the discovery of the DNA double helix by Channel 4 in the UK, then later in the United States on PBS early in 2004. The two DVDs in this package are the Channel 4 (PAL format) versions, with a British narrator.
Work with DNA is central to many, if not most, developments in modern biotechnology. There is growing public awareness of DNA technologies, their possible applications and wider implications. However, much of the essential debate about current genetics has generated more heat than light.
Fortunately, the basic science upon which DNA technologies are founded features in nearly every school examination syllabus in biology or science. This booklet of nine practical protocols plus safety and background information is intended primarily for post-16 students of biology and their teachers.
The practical exercises described in 'Illuminating DNA' provide an introduction to some of the classical techniques of molecular biology in a form suitable for the school laboratory. For reasons of safety and expense, some the work in this booklet is not particularly suited to open-ended practical investigations, but some ideas that may provide starting points for additional work of your own are given. More ideas are provided in other NCBE publications such as: The Lambda DNA Protocol; Investigating Plant DNA; Nature's Dice; and The Transformer Protocol. The latter publication also provides an introduction to ethical, social and other issues raised by DNA technology.
An adequate treatment of the wider issues raised by DNA technology (particularly those associated with human genetics, environmental concerns or food biotechnology) cannot be given in this slim booklet, nor can it take the place of the many excellent school textbooks covering basic biochemistry and molecular biology. It aims merely to provide sufficient information for readers to understand the practical exercises. References to other materials are provided throughout the publication.
In 1868, Johann Friedrich Miescher travelled from his native Switzerland to Tübingen in Germany. The 24-year-old had come to study in the laboratory of Ernst Felix Hoppe-Seyler, a pioneering biochemist who coined the modern name for the red pigment in blood, haemoglobin. After several months of toil in a laboratory in the cellar of Tübingen Castle, Miescher managed to isolate a previously-unknown acidic substance from white blood cells (leucocytes) washed from pus-laden bandages donated by a nearby hospital. Miescher called his discovery 'nuclein' because it was found in the nuclei of the cells. This substance was impure however, and Hoppe-Seyler insisted on repeating the work himself before he would allow an account to be published in his newly-formed journal of biochemistry.
Dean Madden tests safer alternatives to ethidium bromide for staining DNA on electrophoresis gels.
The stains under test were: