When the enzyme cellulase is added to a viscous solution of soluble cellulose (CMC), the viscosity of the solution is dramatically reduced. This change in viscosity can readily be measured, by timing how long the solution takes to drain through a syringe barrel. If the CMC solution is made up using buffers of various pHs (e.g., pH 4, 7 and 9), the effect of pH on the enzyme’s activity can easily be investigated. This convenient pack contains cellulase, carboxymethylcellulose (CMC) and full range pH indicator. (The pH indicator is simply added to the various CMC solutions, to ‘colour-code’ them and avoid confusion.)

 

PACK CONTENTS

This pack contains 100 g of GPR Rectapur carboxymethylcellulose, 50 mL of Full range pH indicator and 100 mL of Novozymes Celluclast®. These items are also available individually.

 

ENZYME ACTIVITY

Celluclast® catalyses the breakdown of the glucose polymers that comprise cellulose to glucose, cellobiose (i.e., pairs of glucose units) and longer chains of glucose units. For practical purposes, the optimum conditions for activity of this enzyme preparation are in the range pH 4.5–6.0, and about 50–60 °C. For further details please refer to the data sheet provided with the enzyme preparation.

 

STORAGE

Generally at a storage temperature of 25 °C, you can expect the enzyme to maintain its declared activity for at least 3 months. At lower temperatures (5–10 °C), the shelf life is considerably increased. If stored correctly, the other items in the pack can be kept for many years.

 

SAFETY

GHS08 pictogram

DANGER. The enzyme in this pack may cause allergy or asthma symptoms or breathing difficulties if inhaled. See the Safety Data Sheet for further advice.

 

CELLULASE ACTIVITY PACK

Cellulase activity pack .......... £36.50 (GBP)

 

INDIVIDUAL ITEMS

Carboxymethylcellulose (CMC) ........ 100 g ........ £16.00 (GBP)

Full range pH indicator (pH 1–13) ........ 50 mL ........ £12.00 (GBP)

Celluclast® ........ 100 mL ........ £17.00 (GBP)

Note: Because the pH indicator is a flammable liquid we cannot send it using Royal Mail. There will therefore be a £5.50 courier charge to most parts of the UK in addition to the price quoted here. Beyond the island of Great Britain and to Northern Scotland the courier charge will be more — please enquire for details when you place your order).

 

ORDERING

All of the prices on this page are in GBP and do not include Value Added Tax (VAT). This tax applies within the European Union only. Postage and handling must also be paid on orders from outside the United Kingdom. Details of how to order are given on the price list and on the Ordering web page.

Please note: We are unable to supply this product to addresses in the United States of America.

Copyright © NCBE, University of Reading, 2018

Enzymes for education

Cellulase activity pack

A cellulose molecule, yesterday.

Model of cellulose molecule RUdevice

PLEASE NOTE
The NCBE supplies these enzymes to schools and colleges for educational use only. Commercial users of enzymes or those who require enzymes for research purposes should contact the enzyme manufacturers or other suppliers, not the NCBE.

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