For more than a quarter of a century this enzyme has been helping those in schools to extract DNA from onions. Don't be fooled by people who say that kiwi fruit provide a less smelly alternative: the gooey stuff you extract from them is mainly pectin.
Neutrase® is a bacterial protease produced by a selected strain of Bacillus amyloliquefaciens. It will break down proteins to peptides.
Neutrase® is used commercially to 'upgrade' proteins of animal and vegetable origin, such as softening wheat gluten. It can also be used to remove the ‘stickwater’ around fish roe.
Neutrase® can be used to degrade proteins when preparing crude extracts of DNA from plant and animal cells (see link to the protocol on the left).
Neutrase® can also be used instead of trypsin in the well-known school practical where skimmed milk is clarified by addition of the enzyme (use 10 mL of 3% w/v dried skimmed milk powder and add 1 mL of 10% v/v Neutrase® solution to it. If the reaction is too fast, simply reduce the amount of Neutrase®).
If you wish to demonstrate the denaturation of an enzyme by heating, Neutrase® is a good one to use, but you do need to heat it to 85 °C for about 2 minutes to see this.
See also: Practical Biology. Quantitative food test. Protein content of powdered milk.
As its name implies, Neutrase® is a neutral protease with an optimum activity around pH 5.5–7.5 and 30–55 °C. It is a metallo- proteinase, requiring zinc ions for its activity. Consequently, it is stabilised by the presence of calcium ions and inhibited by EDTA. The stability of Neutrase® at a particular temperature is influenced by the type and concentration of the proteins present.
If it is stored at 3–5 °C, Neutrase® will retain its declared activity for at least a year. Do not freeze.
DANGER. May cause allergy or asthma symptoms or breathing difficulties if inhaled. See Safety Data Sheet for further advice.
Neutrase® ..... 100 mL ..... £19.00 (GBP)
All of the prices on this page are in GBP and do not include Value Added Tax (VAT). This tax applies within the European Union only. Postage and handling must also be paid on orders from outside the United Kingdom. Details of how to order are given on the price list and on the Ordering web page.
Enzymes for education
Neutrase is often used in school for extracting DNA from onions.
GENERAL SAFETY ADVICE, ETC
Safety data sheet
The NCBE supplies these enzymes to schools and colleges for educational use only. Commercial users of enzymes or those who require enzymes for research purposes should contact the enzyme manufacturers or other suppliers, not the NCBE.