This module allows students to amplify chloroplast DNA using the polymerase chain reaction (PCR). The length of the fragments produced can be used to infer evolutionary relationships.
ABOUT THIS MODULE
The polymerase chain reaction (PCR) is one of the most important and powerful methods in molecular biology. It enables millions of copies of specific DNA sequences to be made easily and quickly. The technique and variations of it are used extensively in medicine, in molecular genetics and in pure research.
This practical kit provides materials for the simple extraction of chloroplast DNA from plant tissue, its amplification by the PCR, and gel electrophoresis of the PCR product. Students can use plants of their choice and identify possible evolutionary relationships between different species. This mirrors the molecular methods used in modern plant taxonomy. This activity presents an ideal opportunity for open-ended investigations by individual students or groups.
This protocol was developed in association with Science and Plants for Schools.
The module contains the consumable materials for the extraction, amplification, and gel electrophoresis of 16 chloroplast DNA samples plus one negative control. The kit includes:
YOU WILL ALSO NEED
For a full list of replacement items, including Students’ booklets, please refer to the Spare parts web page.
Please note that once you have a Base Unit, the most cost-effective option is probably to buy the Investigating plant evolution module rather than individual replacement items.
THE PCR AND PLANT EVOLUTION MODULE
The PCR and plant evolution module .....£163.00 (GBP)
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