DNA science and technology
DNA
Darkbluestrip
University of Reading
NCBE

This module allows students to amplify chloroplast DNA using the polymerase chain reaction (PCR). The length of the fragments produced can be used to infer evolutionary relationships.

ABOUT THIS MODULE

The polymerase chain reaction (PCR) is one of the most important and powerful methods in molecular biology. It enables millions of copies of specific DNA sequences to be made easily and quickly. The technique and variations of it are used extensively in medicine, in molecular genetics and in pure research.

This practical kit provides materials for the simple extraction of chloroplast DNA from plant tissue, its amplification by the PCR, and gel electrophoresis of the PCR product. Students can use plants of their choice and identify possible evolutionary relationships between different species. This mirrors the molecular methods used in modern plant taxonomy. This activity presents an ideal opportunity for open-ended investigations by individual students or groups.

This protocol was developed in association with Science and Plants for Schools.

MODULE CONTENTS

The module contains the consumable materials for the extraction, amplification, and gel electrophoresis of 16 chloroplast DNA samples plus one negative control. The kit includes:

YOU WILL ALSO NEED

REPLACEMENT ITEMS

For a full list of replacement items, including Students’ booklets, please refer to the Spare parts web page.

Please note that once you have a Base Unit, the most cost-effective option is probably to buy the Investigating plant evolution module rather than individual replacement items.

THE PCR AND PLANT EVOLUTION MODULE

The PCR and plant evolution module .....£163.00 (GBP)

ORDERING

All of the prices on this page are in GBP and do not include Value Added Tax (VAT). This tax applies within the European Union only. Postage and handling must also be paid on orders from outside the United Kingdom. Details of how to order are given on the price list and on the Ordering web page.

Please note: For legal reasons, we cannot accept orders by eMail.

 

Electrophoresis modules

Electrophoresis resources

Other DNA items

PDFDOWNLOADABLE PDFs

Instructions

Safety Data Sheets



Equipment and materials | DNA
Equipment and materials | DNA