The NCBE’s electrophoresis equipment can be used to analyse proteins. You do, however, need a special type of agarose to carry out this work.

ABOUT THIS MODULE

Small samples of protein-containing foods (e.g., fish or nuts) are mixed with Laemmli buffer. This extracts the proteins and gives them a negative electrical charge. The samples are separated by electrophoresis, then the gel is stained and de-stained to reveal the proteins.

MODULE CONTENTS

This module contains sufficient materials for the analysis of 96 protein samples.

• 8 pieces of carbon fibre electrode tissue
• 6 g high-grade agarose, suitable for electrophoresis of proteins
• 50 mL TB electrophoresis buffer (a 10x concentrate)
• 50 mL Laemmli buffer
• 2 x 100 mL Colloidal Coomassie Blue stain
• 100 multi-coloured 1.5 mL microcentrifuge tubes
• 100 micropipette tips
• 8 1 mL plastic syringes
• 3 mL SDS solution (10%)
• Approx. 100 mm 5 mm dia. silicone rubber tubing
• 8 full-colour students’ guides
• 1 full-colour teacher’s guide

YOU WILL ALSO NEED

• A Base Unit, with electrophoresis tanks, combs and microsyringes
• A 36 V mains transformer
• Protein samples (almonds and fish can give good results)

REPLACEMENT ITEMS

For a full list of replacement items, including Students’ booklets, please refer to the Spare parts web page.

Please note that once you have the Base Unit or a Science Year Protein Kit (see below), the most economical option is probably to buy a Protein Power! Module.

PROTEIN POWER MODULE

Protein power module .....£72.00 (GBP)

THE SCIENCE YEAR PROTEIN KIT

During the UK’s ‘Science Year’ in 2002, the NCBE provided a special one-off kit containing the materials from the Protein Power module plus eight sets of electrophoresis equipment. Unlike that free kit, current Protein power module does not contain any electrophoresis equipment.

ORDERING

All the prices on this page are in GBP and do not include Value Added Tax (VAT). This tax applies within the European Union only. Postage and handling must also be paid on orders from outside the United Kingdom. Details of how to order are given on the price list and on the Ordering web page.

Please note: For legal reasons, we cannot accept orders by eMail.

 

Electrophoresis modules

The Lambda protocol
Nature’s dice
Plant evolution PCR
Protein power!

Electrophoresis resources

Base unit
36 V mains transformer
Microcentrifuge
Micropipettes
Spare parts

Other DNA items

Bacterial transformation kit
DNA pendant kit

DOWNLOADABLE PDFs

NCBE Price list

Instructions

Teacher’s guide
Student’s guide

 

PRESENTATIONS

PowerPoint presentation
Keynote presentation