Equipment and materials | DNA | Bacterial transformation

Genetic modification is an essential technique in research, medicine and plant breeding. This new kit with green fluorescent protein is designed for use by 14–16 year-old students.

ABOUT THIS KIT

This practical kit provides:

a safe practical demonstration that DNA really is the genetic material;
practical experience of one of the key techniques used in genetic modification;
an opportunity to understand and carry out basic microbiological techniques;
an opportunity for older students to plan and carry out practical investigations of their own.

KIT CONTENTS

The kit contains sufficient materials for 18 students working individually or 36 working in pairs.

Sterile Petri dishes (20)
Microcentrifuge tube holder
Sterile transformation buffer
Sterile plasmid DNA
Microwavable agar containing kanamycin
Coloured 1.5 mL microcentrifuge tubes (18)
A slope culture of E. coli (Strain TG2)
Sterile 'Pastettes' (26)
Sterile inoculation loops (20)
Sterile spreaders (18)
Students' guides (18)
Teacher's guide
Ultraviolet keyring torches (2)

Please note that the sterile Pastettes, inoculation loops and spreaders are single use plastic items. They are not autoclavable and cannot be re-used.

YOU WILL ALSO NEED

Lab facilities suitable for microbiology, including bunsen burners and laboratory coats
An incubator that can be set to 37 °C
An autoclave
Waste pots and disinfectant, such as 'Virkon'

REPLACEMENT ITEMS

The following replacement parts are available for the Bacterial transformation kit:

E. coli K-12 TG2 (slope culture) ..... £12.00 (GBP)
pNCBE-Kan-GFP plasmid DNA (200 µL) ..... £12.50 (GBP)
Transformation buffer (4 mL) ..... £8.00 (GBP)
Kanamycin/X-Gal/LB agar (1 sachet) and LB agar (1 sachet) ..... £14.00 (GBP)
Ultraviolet keyring torches (2) ..... £9.00 (GBP)
Students' guides (18) ......*£10.00 (GBP)

BACTERIAL TRANSFORMATION KIT

Bacterial transformation kit ....£65.00 (GBP)

DESPATCH

Because the plasmid DNA in this kit should be stored frozen, kits will be dispatched by first class post, Monday-Thursday only.

Please note that for safety reasons this kit is not generally available outside the UK (please enquire for details).

ORDERING

All of the prices on this page are in GBP and do not include Value Added Tax (VAT). This tax applies within the European Union only. Postage and handling must also be paid on orders from outside the United Kingdom. Details of how to order are given on the price list and on the Ordering web page.

Please note: For legal reasons, we cannot accept orders by eMail.