
The NCBE supplies a range of high-quality enzymes from Novozymes and DSM, exclusively for educational use.
HOW LONG CAN I STORE THEM FOR?
The concentrated enzyme solutions or powders come in re-sealable plastic bottles and are accompanied by product specification sheets and safety information. The data sheets describe industrial uses of the enzymes and provide important information regarding their stability, dosage rates and optimum working conditions. If stored in a refrigerator at 3-5 °C most of these enzymes will retain their declared activity for many months.
WHAT CONCENTRATION ARE THEY?
We are asked this question every week. For enzymes, ‘concentration’ is a fairly meaningless measurement: the activity of an enzyme depends upon many factors, such as the pH, the substrate being used and its concentration, whether co-factors or inhibitors are present, the temperature and so on.
Usually, in a school laboratory context, teachers will be interested in achieving results rapidly so that the work can be completed within, say, a single lesson. This means that the amount of enzyme product used will often be far greater than that which is typical in industry.
For example, in industry, about 130 mL of Pectinex is used per tonne of apples to maximise juice extraction; in the classroom, 1 mL per 50 g of apple is normally used to obtain results within a 30-minute period.
In short, we cannot answer the question ‘what concentration should I use?’ Instead, we recommend that you study the data sheet provided and test the particular protocol you have in mind before attempting it with students.
Additional advice is given in the ‘Using enzymes’ document, which can be downloaded from the menu on the right.
Please note that for regulatory reasons, we cannot supply these enzymes to addresses in the United States of America.
Copyright © NCBE, 2012 | www.ncbe.reading.ac.uk