Protease | Neutrase®

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Alpha-amylase

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Amyloglucosidase

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Cellulase

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Carbohydrase mix

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Glucose isomerase

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Invertase

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Lactase

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Pectinase for juicing

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Pectinase for peeling

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Pectin esterase

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Protease

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Catalase

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Chymosin

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Fungal 'rennin'

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'Detergent' enzymes

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Special value packs

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Using enzymes

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Price list 2008
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DNA your onions?

Thousands do, because for over a decade this enzyme has been helping those in schools to extract DNA from onions.

Don't be fooled by people who say that kiwi fruit provide a less smelly alternative -- the gooey stuff you extract from them is simply pectin.

The latest trick, however, is to extract DNA from 'caviar'. Read all about it below.

SOURCE

Neutrase is a bacterial protease produced by a selected strain of Bacillus amyloliquefaciens. It will break down proteins to peptides.

Safety guidelines for practical work with enzymes in schools
can be found here.

USES

Neutrase is used commercially to 'upgrade' proteins of animal and vegetable origin. We use it, however, to degrade proteins when preparing crude extracts of DNA from plant and animal cells.

IMPORTANT NOTE

The NCBE supplies these enzymes to schools and colleges for educational use. Commercial users of enzymes or those requiring enzymes for research purposes should contact the enzyme manufacturers, not the NCBE.

ACTIVITY

As its name implies, Neutrase is a neutral protease with an optimum activity around pH 5.5-7.5 and 45-55 °C. It is a metallo- proteinase, requiring Zn ions for its activity. Consequently, it is stabilised by the presence of Ca2+ ions and inhibited by EDTA.

The stability of Neutrase at a certain temperature is influenced by the type and concentration of the proteins present. Neutrase can be inactivated by heat treatment e.g., 2 minutes at 85 °C.

Novozymes

STORAGE

If it is stored at 3-5 °C, Neutrase will retain its declared activity for at least a year.

EXTRACTING DNA

The on-line journal Bioscience Explained has a simple method of extracting DNA from 'caviar'. DNA extracted in this way can be 'run' on an electrophoresis gel to produce a dramatic 'smear' of DNA fragments. Instructions for isolating DNA from frozen peas can also be found here.

PROTEASE

Protease (Neutrase)..... 100 mL ..... £9.00 (GBP)

Please note: All the prices on this page are in GBP and do not include Value Added Tax (VAT). This tax applies within the European Union only. Postage and packing must also be paid on orders from outside the United Kingdom.

Please note: We do not currently accept orders via eMail.

Copyright © National Centre for Biotechnology Education, 2008 | www.ncbe.reading.ac.uk