| ||
| | |
 | ||
| | |
 | ||
| | |
 | |||||
DOWNLOAD | |||||
| |||||
| | | | | |
CUTTING & PASTING DNA |  | |||||
 | How to download these files Illuminating DNA is available as downloadable Adobe Acrobat (PDF) files. Details of how to obtain a free copy of the Adobe Acrobat Reader software are given here. | | ||||
T4 DNA ligase can be used to join double-stranded DNA fragments that have staggered or blunt ends, produced by the action of a restriction enzyme. Here the enzyme EcoRI is used to sever plasmid DNA which is then re-joined using DNA ligase. The resulting fragments are separated by gel electrophoresis. The ligation reaction is only permitted to proceed for two minutes, so that it is incomplete. Consequently, when the cut and ligated plasmid DNA is run on an electrophoresis gel, a pattern of bands is produced, with fragments that vary in size according to the number of fragments that have been joined together. | ||||||
 | ||||||
| | | | | | |
Copyright © National Centre for Biotechnology Education, 2006 | www.ncbe.reading.ac.uk | ||
| | |
