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PLASMID EXTRACTION THE HARD WAY |  | |||||
 | How to download these files Illuminating DNA is available as downloadable Adobe Acrobat (PDF) files. Details of how to obtain a free copy of the Adobe Acrobat Reader software are given here. | | ||||
This is the 'classical' method of isolating plasmid DNA; with it you can see the purpose of each step. The method requires the use of a high-speed microcentrifuge. Alternative methods that use small resin filters that nest within a microcentrifuge tube are also available as kits from suppliers of materials for molecular biology, but these tend to be too costly for everyday teaching purposes. Instructions are also given for running the extracted plasmid DNA on an electrophoresis gel, to reveal 'relaxed', linear or supercoiled plasmids. | ||||||
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Copyright © National Centre for Biotechnology Education, 2006 | www.ncbe.reading.ac.uk | ||
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