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DIPHENYLAMINE REACTION TO DETECT DNA |  | |||||
 | How to download these files Illuminating DNA is available as downloadable Adobe Acrobat (PDF) files. Details of how to obtain a free copy of the Adobe Acrobat Reader software are given here. | | ||||
A standard method of measuring the amount of DNA in a solution is to measure its absorbance of ultraviolet light. Most schools do not have a UV spectrophotometer however, so the following method, which uses a colorimeter capable of measuring absorbance at 660 nm, can be used instead. For this assay, denatured DNA (strictly, deoxypentose) is reacted with acidified diphenylamine to produce a dark blue-coloured compound. Both a quick qualitative method and a slower, though more accurate, quantitative method are described in this section. A reference is also given to an article describing DNA quantification on agarose gels, which may be used where distinct fragments of DNA are present. | ||||||
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Copyright © National Centre for Biotechnology Education, 2006 | www.ncbe.reading.ac.uk | ||
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