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EQUIPMENT & MATERIALS | ||
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In this booklet we have tried to describe practical protocols that are relatively inexpensive and require little specialist equipment. The NCBE endeavours to develop and supply at low cost several of the items required, such as electrophoresis apparatus, restriction enzymes, plasmids and so on. Because the details of suppliers and their products change frquently (no fewer than six of the names and addresses below have changed in the last two years!), we have decided not to list them in this booklet. Some of the investigations described here require a high-speed microcentrifuge. An inexpensive, award-winning microcentrifuge for school use has been developed by our friend Professor John Cave of Teaching Resources at Middlesex University and the Technology Enhancement Programme (TEP), with help from Science and Plants for Schools (SAPS) and the NCBE. This is now available to UK schools from the NCBE. | ||
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SUPPLIERS | ||
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National Centre for Biotechnology Education T: 0118 9873 743 Equipment for DNA gel electrophoresis. Dried restriction enzymes EcoRI, BamHI and HindIII; Lambda DNA, and 1 Kb DNA ladder. Buffers, DNA stains, etc. Kit for bacterial transformation.
Middlesex University Teaching Resources T: 0845 6014773 (Orders) Inexpensive 12-volt microcentrifuge for school use.
Carolina Biological Supply Company T: +1 336 584 0381 A wide range of materials and equipment for schools, including a special biotechnology catalogue. N.B. The use of some of the bacterial transformation kits sold by Carolina and other US suppliers may not be permitted within the European Union.
Alpha Laboratories Ltd. T: 01703 483000 Microcentifuge tubes, screw-cap tubes, sterile pastettes.
Amersham Biosciences UK Ltd. T: 0800 515 313 DMF, antibiotics, LB media, PIPES, X-Gal.
Fisher Scientific UK T: 01509 231166 (Sales) General laboratory supplier, sterile loops and spreaders.
Merck Ltd. (also BDH) T: 0800 22 33 44 (Sales) General laboratory supplier, with a large range, including chemicals and disposable equipment such as loops and spreaders.
Sigma-Aldrich Company Ltd. T: 01202 733114 Comprehensive range of products for molecular biology, including: DMF; antibiotics; LB media in tablet and powdered form; X-Gal.
Philip Harris Education (now part of the Findel group) T: 0845 120 4520 General laboratory supplier, disinfectants.
Promega UK Ltd. T: 0800 378994 Range of products for molecular biology.! | ||
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Copyright © National Centre for Biotechnology Education, 2006 | www.ncbe.reading.ac.uk | ||
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