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MICROBIAL MODIFICATION |  | |||||
 | How to download these files Illuminating DNA is available as downloadable Adobe Acrobat (PDF) files. Details of how to obtain a free copy of the Adobe Acrobat Reader software are given here. | | ||||
Here is a 'generic' method of transforming cells; it will work with most 'cloning' strains of bacteria and plasmids. Because colonies are taken directly from Petri dish cultures (rather than liquid cultures at the optimum phase of growth), the protocol uses a specially-devised transformation buffer to ensure greater transformation efficiency.This 'self-cloning' protocol is for plasmids that have the lacZ gene (and hosts that lack it) so that X-Gal can be used as a marker. This protocol is similar to that used in a practical kit from the NCBE. The Transformer Protocol also includes a role play exercise so that students can more easily consider the ethical, environmental and social issues that arise from genetic modification. | ||||||
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Copyright © National Centre for Biotechnology Education, 2006 | www.ncbe.reading.ac.uk | ||
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