Practical biotechnology
Cloned cauliflower

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CLONED CAULIFLOWER

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Landmark publication

A single publication was almost wholly responsible for the development of plant tissue culture in UK schools. Tony Storr's 'Plant tissue culture' was published as part of a series of industry-sponsored booklets by the Association for Science Education. This gave details of how to clone tissue from a variety of plants. A key feature was the use of a home-made transfer cabinet to reduce the risk of contamination. This idea was later taken up by similar German educational publication.

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In the brief period after the introduction of the GCSE examination in England and Wales, but before the National Curriculum came fully into force, biotechnology education flourished. Practical experience of plant tissue culture was called for in many biology and some science syllabuses - notably in Suffolk Science in its first incarnation.

The plant most commonly-used for such work in schools and colleges was cauliflower. Carrots were sometimes tried, but it was difficult to isolate the meristematic tissue from the tap root as suggested. Cauliflower, in contrast, proved ideal for plant tissue culture, because it is readily-available, robust enough to withstand the rigours of being handled by students and grows rapidly. Growth can be seen after 10 days and plantlets ready for transplantation are formed within 12 weeks.

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ADDITIONAL RESOURCES

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Plant Tissue Culture Information Exchange
(Texas A&M University)

PDF

You can download a facsimile of the original (1985) version of Tony Storr's booklet 'Plant tissue culture' from here as a PDF file (1.8 Mb).

Copyright © National Centre for Biotechnology Education, 2006 | www.ncbe.reading.ac.uk