Practical protocols
Illuminating DNA

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The Lambda DNA protocol

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The Transformer protocol

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Illuminating DNA

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In a jam and out of juice

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Practical fermentation

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Practical biotechnology

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Nature's dice

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Protein power!

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Plant tissue culture

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Unpublished protocols

First edition, 2000.

This is one of a series of booklets that is intended to replace the NCBE's popular 'Practical biotechnology' guide, which is now out-of-print.

It's a 44-page, A4 full-colour booklet with illustrated instructions for nine practical activities using DNA, ideas for additional investigations, safety guidelines and a 10-page introduction to molecular genetics for students.

A printed version of this booklet can be purchased from the NCBE, and you may download the booklet as a series of PDF files (free-of-charge) if you wish.

The production of this booklet was supported by Unilever plc.

ISBN: 0 7049 1370 4

Safety guidelines for practical work with DNA
can be found here.

THE PROTOCOLS

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Introduction
cover, introduction, acknowledgements, copyright notice and contents page

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DNA: Molecule of the century
background information

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Safety guidelines
good microbiological practice

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Modelling DNA
physical and computer models are useful for studying molecular structure

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Extracting DNA
from onions and other tissues

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DNA quantification
diphenylamine gives a blue-coloured complex with deoxyribose

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Induction of beta-galactosidase in E.coli
E. coli makes the enzyme beta-galactosidase - but only when induced to

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Extraction of plasmid DNA
plasmid DNA can be extracted from bacteria and run on an electrophoresis gel

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Bacterial transformation
prove that DNA is the genetic material by transforming bacteria

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Restriction and ligation of DNA
restriction enzymes and DNA ligase can be used to 'cut and paste' DNA

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Restriction site mapping of lambda DNA
find out where the restriction sites are in phage lambda's DNA

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Amplifying lambda DNA
using the polymerase chain reaction

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Ideas for further investigations

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Running gels | Units of measurement
hints for electrophoresis of DNA on agarose gels

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DNA revealed
alternatives to ethidium bromide for staining DNA

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Recipes
for media, buffers,
etc.

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Glossary

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Other sources of information

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Suppliers
of the necessary equipment and materials

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Curriculum links

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How to download these files

Illuminating DNA is available as downloadable Adobe Acrobat (PDF) files. Just click on a title to the left to go to the download pages.

Details of how to obtain a free copy of the Adobe Acrobat Reader software are given here.

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Copyright National Centre for Biotechnology Education, 2008 | www.ncbe.reading.ac.uk