Microbiology | Recommended microbes
Fungi

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Fungi

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FUNGUS

EDUCATIONAL USE, INTEREST
and SUITABILITY

EASE OF USE  and MAINTENANCE

Agaricus bisporus

Edible mushroom; useful for a variety of investigations on factors affecting growth.

Grows on compost containing well-rotten horse manure; available as growing 'kits'.

Armillaria mellea

The honey fungus; causes decay of timber and tree stumps. Produces rhizomorphs.

Grows very well on malt agar. Some authorities recommend carrot agar.

Aspergillus nidulans [1]

For studies of nutritional mutants. Produces abundant, easily-dispersed spores - may become a major laboratory contaminant!

Grows on Czapek Dox yeast agar. Special media required for studying nutritional mutants.

Aspergillus niger [1]

Useful for studies of the influence of magnesium on growth and the development of spore colour. Used commercially for the production of citric acid. Produces abundant, easily-dispersed spores - may become a major laboratory contaminant!

Requires special sporulation medium for some investigations.

Aspergillus oryzae [1]

Produces a potent amylase; useful for studies of starch digestion. Also produces protease. Used in the production of rice wine (saki) in Japan.

Grows on malt agar; add starch (or protein) for investigations of enzyme activity.

Botrytis cinerea

Causes rotting in fruits, particularly strawberries. Useful for studies of Koch's postulates with fruit, vegetables and Pelargonium sp. Important in the production of some dessert wines ('noble' rot).

Can be grown on malt agar or agar with oatmeal.

Botrytis fabae

Causes disease in bean plants.

Requires agar with oatmeal.

Chaetomium globosum

Useful for studies of cellulase production; thrives on paper.

Can be grown on V8 medium but survives well just on double thickness wallpaper, coated with a flour paste.

Coprinus lagopus

For studies of fungal genetics.

In the wild, grows on horse dung.

Eurotium ( = Aspergillus) repens

Produces yellow cleistocarps (cleistothecia) embedded in the medium and green conidial heads in the same culture.

Requires special medium.

Fusarium graminearum

Causes red rust on wheat; used in the manufacture of 'Quorn' mycoprotein.

Can be grown on V8 medium.

Fusarium oxysporum

A pathogen of many plants. Produces sickle-cell shaped spores, a red pigment and pectinase.

Grows well on several media including malt, potato dextrose and Czapek Dox yeast agar.

Fusarium solani

Digests cellulose; macroconidia have a sickle shape.

Grows on potato dextrose agar.

Helminthosporium avenae

A pathogen of oats.

May not grow easily in laboratory cultures.

Kluyveromyces lactis

A yeast isolated from cheese and creamery products. Ferments lactose and used to convert dairy products to lactose-free forms. Genetically-modified strains are used to produce chymosin (rennin).

Grows on malt agar or glucose nutrient agar.

Leptosphaeria maculans

For studies of disease in brassica plants.

Requires cornmeal agar or prune yeast lactose agar to promote sporulation in older cultures.

Monilinia ( = Sclerotinia) fructigena

For studies of brown rot in apples. Useful for studies of Koch's postulates.

Grows on malt agar or potato dextrose agar.

Mucor genevensis

For studies of sexual reproduction in a homothallic strain of fungus.

Grows on malt agar.

Mucor hiemalis

For studies of sexual reproduction between heterothallic + and - strains and zygospore production.

Grows on malt agar.

Mucor mucedo

Common black 'pin mould' on bread. For sporangia (asexual), mating types and amylase production.

Grows on malt agar.

Myrothecium verucaria

For studies of cellulose decomposition but Chaetomium globosum is preferred.

Grows on malt agar.

Neurospora crassa [1]

Red bread mould. Produces different-coloured ascospores. Can be used in studies of genetics. Beware - readily becomes a major laboratory contaminant!

Grows on malt agar.

Penicillium chrysogenum [1]

Produces penicillin; useful for comparative growth inhibition studies in liquid media or when inoculated onto agar plates seeded with Gram-positive and negative bacteria. Produces yellow pigment.

Grows on malt agar, though some authorities indicate that it thrives better on liquid media.

Penicillium expansum [1]

Does not produce penicillin; causes disease in apples. Useful for studies of Koch's postulates.

Grows on malt agar.

Penicillium notatum [1]

Produces penicillin; useful for comparative growth inhibition studies in liquid media or when inoculated onto agar plates seeded with Gram-positive and negative bacteria.

Grows on malt agar.

Penicillium roqueforti [1]

Does not produce penicillin; the familiar mould of blue-veined cheese.

Grows on malt agar.

Penicillium wortmanii [1]

Produce wortmin rather than penicillin.

Grows on malt agar.

Phaffia rhodozyma

A fermenting red yeast. Used to colour the food supplied to farmed salmon.

Grows on yeast malt agar.

Phycomyces blakesleanus

Produces very long sporangiophores which are strongly phototropic.

Grows on malt agar.

Physalospora obtusa

An ascomycete fungus that grows on apple. Thought to produce pectinase.

Grows on potato dextrose agar.

Phytophthora infestans [2]

Causes potato blight. Produces motile zoospores.

Can be grown on V8 medium.

Plasmodiophora brassicae

For studies of disease in brassica plants, particularly club root. Useful for studies of Koch's postulates.

May not grow easily in culture.

Pleurotus ostreatus

Edible oyster cap mushroom.

Can be grown on rolls of toilet paper!

Pythium de baryanum [2]

Causes 'damping off' of seedlings; cress is best to use.

Grows on cornmeal agar.

Rhizopus oligosporus

Used in the fermentation of soya beans to make tempe, a food consumed in Indonesia.

Grows on potato dextrose agar, Czapek Dox yeast agar and other fungal media.

Rhizopus sexualis

Produces rhizoids and zygospores. Useful for studies of the linear growth of fungi.

Grows on potato dextrose agar and other fungal media.

Rhizopus stolonifer

Produces rhizoids. Produces lipase.

Grows on potato dextrose agar, potato carrot agar, Czapek Dox yeast agar and other fungal media.

Rhytisma acerinum

An indicator of air pollution: less common in polluted areas. On sycamore leaves, it forms 'tar' spot lesions, the number or diameter of which can be compared at different sites.

Difficult to maintain but laboratory cultures are not likely to be needed.

Saccharomyces cerevisiae

Valuable for work in baking and brewing, showing budding, for spontaneous mutation and mutation-induction experiments, and for gene complementation using adenine- and histidine-requiring strains.

Grows on malt agar or glucose nutrient agar.

Saccharomyces diastaticus

Able to grow on starch by producing glucoamylase.

Grows on malt agar and nutrient agar + 1% starch.

Saccharomyces ellipsoideus

Used in fermentations to produce wine; can tolerate relatively high concentrations of ethanol.

Grows on malt agar.

Saprolegnia litoralis [1]

Parasitic on animals. Produces zoospores. Good illustration of asexual and sexual stages.

Culture by baiting pond water with hemp seeds.

Schizosaccharomyces pombe

Large cells, dividing by binary fission. Good for studies of population growth, using a haemocytometer for cell counts. Prone to thermal shock.

Grows on malt agar. For studies of population growth, a malt extract broth can be used.

Sordaria brevicollis

For studies of fungal genetics, including inheritance of spore colour and crossing over in meiosis.

Requires special medium for crosses between strains.

Sordaria fimicola

For studies of fungal genetics, including inheritance of spore colour and crossing over in meiosis.

Grows on cornmeal, malt and other agars but may not transfer readily from one medium to another. White-spore strain may not always grow normally on standard cornmeal agar.

Sporobolomyces sp.

Found on leaf surfaces. Spores are ejected forcibly into the air from mother cells.

Grows on malt, yeast malt and glucose nutrient agar but laboratory cultures may not be needed.

Trichoderma reesei

Used in the commercial production of cellulase.

Grows on malt agar.

NOTES

1.

Possible risk of allergy/asthma if large numbers of spores are inhaled.

2.

These species are now classified as protoctists, so they may not be found in the lists of fungi from suppliers.

Copyright © National Centre for Biotechnology Education, 2006 | www.ncbe.reading.ac.uk